The First Findings on the Differences in Complete Blood Count Between Diploid and Triploid Tench, Tinca tinca L.

Abstract

Svobodova. Z .. 1. Kolarova, M. Flajshans: The First Findings of the Differences in Complete Blood COltllt Between Diploid and Triploid Tench. Tinea tinca L. Acta vet. Bmo 1998, 67: 243-248. The aim of this work was to evaluate differences in complete blood count of diploid and triploid tench and concentration of total protein in blood plasma of these fish. Haematological examination of 78 specimens of three-year-old tench. consisting of 21 triploids and their 57 diploid siblings was performed in July 1996. Analysis of variance test confirmed a significantly lower erythrocyte count in triploids (P < 0.01). as well as a significantly lower haemoglobin content (P < 0.05), significantly higher medium corpuscular volume. higher medium corpuscular haemoglobin and lower medium corpuscular haemoglobin concentration (all at P < 0.01) compared to diploids. The haematocrit value of triploids was insignificantly lower compared to diploids. The differences of erythrocyte nuclear area. perimeter. average size, major axis and aspect ratio between diploids and triploids were found signiticant at P < 0.01 and the effect of ploidy level on these dimensions was considered highly significant (P < 0.000 I). No effect of ploidy level was proven for erythrocyte nuclear minor axis. The leukocyte count of triploids was insignificantly lesser compared to diploids. Triploid tench were found of significantly lower concentration of total protein in blood plasma compared to diploids (P < 0.01). Triploid tench could be hypothesized to have a lower oxygenation capacity of blood. as well as a lower non-specific immunity level compared to diploids. Insigniticantly higher erythrocyte count, haemoglobin content. haematocrit value. and significantly higher concentration of total protein in blood plasma (P < 0.0 I) was reported for diploid females compared to males.