The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey

Abstract

A total of 127 stool samples (rectal swab) from diarrheic calves, up to one mouth of age, were collected during one year period (2014-16) from three cities (Sivas, Malatya and Elazig) located central Turkey. 432 bp partial sequence of Nsp1ab gene were amplified. The PCR amplicons were purified and sequenced and deposited to GenBank. Sequence alignment and phylogenetic analysis being based on partial nucleotide sequences of Nsp1ab gene was constructed. As a result of RT-PCR study, we found that the 3.15% of fecal samples (4/127) were positive for diarrhea calves from Turkey. The neighbor-joinning tree of partial sequence of Nsp1ab gene (ORF1a) indicated that strains were substituted under three distinct lineage. None of our strains belonged to the Neuro1 lineage (lineage 3) of Bovine astrovirus which was isolated from bovine with neurological symptoms. On the other hand, while BAstVHT1-TUR strain was substituted under lineage 1a, the other novel starts were lineage 2. While the identity of analyzed sequences varies from 51.1 to 100%, novel strains were calculated between 75.8 to 100 % each other. As a result, we report the first detection and phylogenetic analysis of Bovine astrovirus from Turkey.