The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1.

Abstract

A xylanase has been found in the archaeon Thermococcus zilligii strain AN1 (DSM 2770), which grows optimally at 75 degrees C. The enzyme had a molecular mass of 95 kDa and a unique N-terminal sequence. It had activity against all five xylans tested and against xylose oligomers, but not against other carbohydrate polymers. The K(m) values found for xylans were typical of those found for bacterial xylanases. The pH optimum for activity was pH 6, and the enzyme half-life at 100 degrees C was 8 min. This is the first description of any archaeal hemicellulase.