Abstract
BackgroundHere, we report the first patient derived hepatitis C virus (HCV) complete genome from Pakistan as is not available from this region of the world.FindingsComprehensive evolutionary and phylogenetic analyses were conducted. The comparison was made in order to identify evolutionary and molecular phylogenetic relationships among HCV strains belonging to genotype 1a. The evolutionary divergence analysis for nucleotide and amino acid sequences, conducted by equal input model, suggested that evolutionary nucleotide and amino acid distances showed that the HCV Pakistani strain was genetically far from Denmark strain (0.29400 nt, 0.819646 aa) and near to German strain (0.06557 nt, 0.139449 aa), respectively.ConclusionThe current study will help to understand phylogenetic relationship of circulating Pakistani isolates.
Highlights
We report the first patient derived hepatitis C virus (HCV) complete genome from Pakistan as is not available from this region of the world
The HCV genome consists of 9.6 Kb, linear, uncapped and single strand RNA
HCV is classified into six main HCV genotypes i.e. 1, 2, 3, 4, 5 and 6 with a genetic variation at nucleotide/amino acid level at 30% [7]
Summary
We report the first patient derived hepatitis C virus (HCV) complete genome from Pakistan as is not available from this region of the world. HCV is classified into genotypes, subtypes, isolates and quasispecies [6]. The present study describes the phylogenetic characterization of complete genome of an HCV isolate belonging to genotype 1a from Pakistan. This study was designed to amplify, clone and sequence genotype 1a cDNA, Pakistan isolate.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
