Abstract
The complete sequence of a novel RNA virus isolated from Tetrastichus brontispae (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins, hypothetical proteins, matrix proteins, glycoproteins, and RNA-dependent RNA polymerases. Sequence analysis indicated that the RNA-dependent RNA polymerase of TbRV-1 shares a 65% nucleotide and 67% amino acid sequence identity with Hubei dimarhabdovirus 2, suggesting that TbRV-1 is a member of the dimarhabdovirus supergroup. This corresponded to the result of the phylogenetic analysis. The affiliation of TbRV-1 with members of the family Rhabdoviridae was further validated by similar transcription termination motifs (GGAACUUUUUUU) to the Drosophila sigmavirus. The prevalence of TbRV-1 in all tissues suggested that the virus was constitutive of, and not specific to, any wasp tissue. To our knowledge, this is the first report on the complete genome sequence of a dimarhabdovirus in parasitoids.
Highlights
Rhabdoviridae, a family of viruses in the Mononegavirales order, are negative-sense (–)single-stranded RNA viruses
The Blast results showed that Tetrastichus brontispae RNA virus 1 (TbRV-1), Tetrastichus brontispae RNA virus 2 (TbRV-2), Tetrastichus brontispae RNA virus 3 (TbRV-3), Tetrastichus brontispae RNA virus 4 (TbRV-4), and Tetrastichus brontispae RNA virus 5 (TbRV-5) were aligned to the viruses that belonged to Rhabdoviridae, Orthomyxoviridae, Iflaviridae, Narnaviridae, and Reoviridae families, respectively
The genome of TbRV-1 exhibited a significant divergence from Diachasminorpha longicaudata rhabdovirus (DlRhV) by Blastn and Blastp, but a higher similarity to two dimarhabdovirus supergroups, HuDV2 and WuIV7 [23,39]
Summary
Rhabdoviridae, a family of viruses in the Mononegavirales order, are negative-sense (–)single-stranded RNA viruses. Based on the strong nexus of virus clustering and the host types, as well as the broad host range of some rhabdovirus, the insects are proposed to be the primary determinants of host range of the plant and animal rhabdovirus [2,3]. 20 officially recognized genera and one viruses are reported [4]. The main insect-associated rhabdoviridae are from the genera Vesiculovirus, Cytorhabdovirus, Nucleorhabdovirus, Sigmavirus, Ephemerovirus and the superfamily dimarhabdovirus [1], where the majority insect vectors are from Diptera, Hemiptera, and Lepidopteara [3]. Among the arthropod-specific viruses, one group of predominantly insect-associated viruses is a sister group to the large clade of plant viruses, while the other group is the sigma virus clade, and are derived from the vector-borne dimarhabdoviruses [3].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
