Abstract
We describe the system design of the first bioluminescence tomography (BLT) system for parallel acquisition of multiple bioluminescent views around a mouse in a number of spectral channels simultaneously. The primary component of this BLT system is a novel mirror module and a unique mouse holder. The mirror module consists of a mounting plate and four mirrors with stages. These mirror stages are right triangular blocks symmetrically arranged and attached to the mounting plate such that the hypotenuse surfaces of the triangular blocks all make 45∘ to the plate surface. The cylindrical/polygonal mouse holder has semitransparent rainbow bands on its side surface for the acquisition of spectrally resolved data. Numerical studies and experiments are performed to demonstrate the feasibility of this system. It is shown that bioluminescent signals collected using our system can produce a similar BLT reconstruction quality while reducing the data acquisition time, as compared to the sequential data acquisition mode.
Highlights
Bioluminescent imaging has proven to be instrumental for studying gene expression, protein interaction, and cellular dynamics in small animal models, and promises to have major impacts on small animal studies towards the development of molecular medicine [1,2,3]
Bioluminescence tomography (BLT) is to reconstruct a bioluminescent source distribution inside a living mouse from optical signals measured on the body surface of the animal [4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20]
Since the mouse holder scheme is the easiest and cheapest, in this paper we focus on this idea to establish the feasibility of this multispectral bioluminescence tomography system design
Summary
Bioluminescent imaging has proven to be instrumental for studying gene expression, protein interaction, and cellular dynamics in small animal models, and promises to have major impacts on small animal studies towards the development of molecular medicine [1,2,3]. To collect multispectral bioluminescent data, optical filters are currently used one at a time in front of a CCD camera lens [18, 20]. The Xenogen IVISTM bioluminescent imaging system 3D series utilizes a rotating mechanism to capture up to eight views one at a time [16]. Another system use a mirror system to collect four views of a mouse, but this system does not allow that the camera system is focused on all the four views, since these views have different distances to the CCD [18].
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