The Fatty Acid Composition of Membrane Lipids in Buds of Silver Birch during the Winter–Spring Period under the Conditions of the Cryolithozone

Abstract

The fatty acid composition of polar lipids, which are the structural and functional bases of cell membranes in the bud of Betula pendula Roth birch, growing under the conditions of the cryolithozone, was studied for the first time. The important role played by lipid metabolism in the winter–spring period, when the apical meristem is at the stage of intrarenal development, was established. It was shown that, during this period, unsaturated fatty acids prevail over saturated in phospholipids and glycolipids in silver birch buds. However, under the conditions of extremely low air temperatures (–40°C and below), the lowest values of double bond index (DBI) and fatty acid unsaturation coefficient (U/S) were recorded. The rise of negative temperatures under the conditions of the cryolithozone (in March) to values usually observed during the winter period throughout the range of silver birch (–20°C and higher) contributed to an increase in the level of membrane lipid unsaturation. It was found that diene fatty acids predominate in phospholipids, and diene and triene acids predominate in glycolipids, the latter reaching maximum values (80.7% of the amount of unsaturated fatty acids) by the beginning of budding. It was hypothesized that, in order to preserve the viability of the apical meristem in buds of silver birch trees under the specific conditions of the cryolithozone, various interrelated adaptive mechanisms were formed. These mechanisms are aimed not only at protecting the rudimentary organs from the effects of sharp fluctuations in daily temperatures during spring, but also at protecting them during winter under conditions of extremely low temperatures (down to –60°С), which do not occur in other parts of its range. One of these mechanisms appeared to be nonspecific, and it was associated with an increase in the unsaturation of the fatty acid composition corresponding to the liquid-crystalline state of membrane lipids. Another adaptive mechanism is probably aimed at protecting cells from dehydration with the involvement of dehydrin proteins, as well as a number of other hydrophilic cryoprotective compounds against the background of increased viscosity of membrane lipids.