Abstract
The disruption of adenovirus type 7 by formamide was studied using virus with3H-thymidine-labeled DNA. In agreement with previous electron microscopical observations, the degradation of the virus appeared to proceed stepwise, resulting in the formation of viral cores which sedimented about three times slower than intact virus when submitted to rate zonal centrifugation in sucrose gradients. The cores contained about 28% of radioactive label originally present in intact14C-amino acid-labeled adenovirus. DNA enclosed in the viral cores or in intermediate particles formed in the process of degradation was susceptible to pancreatic DNase 1. The size of the DNA fragments obtained after treatment with the enzyme under the described conditions did not exceed 1/600 the size of the viral genome. The degree of viral degradation was dependent on temperature and pH as well as on the formamide concentration.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
