The fat that matters: Soil food web analysis using fatty acids and their carbon stable isotope signature

Abstract

Chemical taxonomy based upon the composition of lipids is widely applied to investigate microbial communities and fatty acids have recently been employed to connect soil microbial and faunal food webs as well as to elucidate functional groups at higher trophic levels. The additional use of compound-specific isotopic analysis of 13 C/ 12 C ratios in fatty acids allows assessing specific trophic links and belowground carbon fluxes. In this review systematic patterns and processes underlying variations in the composition of fatty acids and their 13 C/ 12 C ratio are described. The emphasis is on biomarker fatty acids, their incorporation and modification, effects of pool size, and analytical methods. Further development of the application of fatty acid profiling to soil ecology should include both advances in experimental research and growth of theory. Accordingly, areas in which future experimentation can lead to progress in soil food web analysis are identified. Overall, combining fatty acid biomarker and their isotopic ratios will allow detailed insight into belowground trophic interactions. ► Insight into soil food web structure by lipids. ► Biomarker fatty acids serve as tool to assign trophic groups. ► Compound specific analysis ( 13 C/ 12 C in fatty acids) allows to verify trophic links.