Abstract
Groups of 5-6 ovariectomized ewes were treated with oestradiol-17 beta (100 mg implant) and/or bromocriptine (1 mg; 2 X daily) or with vehicle for 30 days during the anoestrous season. Oestradiol increased (P less than 0.05) the priming effect of 2 X 1 micrograms LH-RH on LH release and decreased (P less than 0.001) pituitary LH content, plasma LH levels, the frequency and amplitude of pulsatile LH release and the LH response to LH-RH. Bromocriptine decreased prolactin levels to less than 2.5 ng/ml (P less than 0.001) and increased the LH response to LH-RH (P less than 0.05). There were no significant treatment interactions. It is concluded that prolactin does not enhance the inhibitory effect of oestradiol on LH synthesis and release in the ewe.
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