Abstract
This paper describes an alternative strategy for the preparation of highly ordered molecularly imprinted mesoporous silica microspheres (MIMSM) for the selective fluorescence sensing of sparfloxacin in biological samples. We designed and synthesized a new functional monomer through the thiol-ene click chemistry reaction of 3-(methacryloyloxy) propyltrimethoxysilane and 3-mercaptopropionic acid. Sparfloxacin was selected as a template, and Mn-doped ZnS quantum dots served as the signal transducing element. This one-pot strategy was used to fabricate the MIMSM. Fourier transform infrared spectroscopy demonstrated successful embedding of a functional monomer in the mesoporous silica microspheres, and the highly ordered, periodic hexagonal mesoporous structure of MIMSM was confirmed with small angle X-ray diffraction, transmission electron microscopy, and N2 adsorption-desorption measurements. The fluorescence intensity of the MIMSM had linear correlation with 0.05–2.0 μg/mL sparfloxacin. There was a rapid response time and excellent selectivity for structural analogues. Under optimal conditions, the limit of detection was 0.012 μg/mL for sparfloxacin. This method was then used with serum samples to achieve recoveries of 88.8–102%.
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