Abstract
The assessment of glutathione (GSH) levels is associated with early diagnostics and pathological analysis for various disorders. Among all kinds of techniques for detecting GSH, the colorimetric assay relying on the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) catalyzed by many nanomaterials with peroxidase-like activity attracts increasing attention owing to its outstanding merits, such as high sensitivity and high selectivity. However, the aggregation between the nanomaterials severely hinders the entrance of TMB into the “active site” of these peroxidase mimics. To address this problem, the D-amino acid incorporated nanoflowers possessing peroxidase-like activity with a diameter of 10–15 μm, TMB and H2O2 were employed to establish the detection system for determining the level of glutathione. The larger diameter size of the hybrid nanoflowers substantially averts the aggregation between them. The results confirm that the hybrid nanoflowers detection system presents a low limit of detection, wide linear range, perfect selectivity, good storage stability and desired operational stability for the detection of GSH relying on the intrinsic peroxidase-like activity and favorable mechanical stability of the hybrid nanoflowers, indicating that the hybrid nanoflowers detection system has tremendous application potential in clinical diagnosis and treatment.
Highlights
When 18 other kinds of amino acids were added to the synthesis system, the same nanomaterials could be seen (Figure S1)
These results suggest that amino acids play a critical role in directing the synthesis of flower-like nanostructures
The slight change in the morphology of the flower-like nanostructures occurs with the change of the amino acid employed, suggesting that the side chain of amino acid is involved in the formation of the acid employed, suggesting that the side chain of amino acid is involved in the formation of the coordination bonds between the amino acid and Cu ion, which is in accordance with the synthesis principle of the incorporated
Summary
The determination of the level of GSH is important for accomplishing the early diagnostics and pathological analysis for these disorders.
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