Abstract
A relatively simple method of extraction and purification of bacterial DNA is described which is suitable for use by sixth-form science students. Centrifugea pellets of actively growing Escherichia coli are lysed in detergent and the histone proteins associated with the bacterial DNA enzymatically digested. The proteins are then eliminated using phenol and trichloromethane extractions. The resulting relatively pure DNA is precipitated in ethanol and used to demonstrate various physical properties of the molecule. The DNA is then hydrolysed to its constituent bases by hot hydrochloric acid. The bases are run against known standards in an ascending chromatogram and subsequently identified under ultraviolet light.
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