The expressions of Wnt2,Wnt5b and Wnt9a in colons of Hirschsprung disease and anorectal malformations

Abstract

Objective To investigate the expressions of Wnt2,WntSb and Wnt9a in tissue samples from the patients with Hirschsprung disease (HD) and anorectal malformations (ARMs).Methods Ninety individuals were recruited in this study,including 30 with HD,30 with ARMs,and 30 controls died of non-gastrointestinal diseases.According to the position of malformations,the 30 ARMs patients were subgrouped into high,intermediate and low ARMs groups.The terminal rectum from the ARMs group and the stenotic and normal appearance intestines from HD patients were collected during operation.The expression levels of Wnt2,Wnt5b and Wnt9a were studied using quantitative Real-time PCR (qRT-PCR) and western blotting.Results In the HD patients,the mRNA of Wnt2,Wnt5b and Wnt9a in stenotic intestine were significantly lower than those in the normal intestine ( the relative quantification of mRNA 10.19 ± 0.27,10.38 ± 0.19,11.43 ± 0.51 vs 18.06 ± 1.07,17.98 ± 1.12,19.99 ± 0.76,P＜0.05).In the ARMs patients,the mRNA of Wnt2,Wnt5b and Wnt9a in high ARMs patients were significantly lower than those in control patients (the relative quantification of mRNA 14.27 ± 0.31,13.69 ± 0.45,13.07 ± 0.67 vs 24.98 ± 2.03,26.43 ± 1.77,28.11 ± 1.44,P＜0.05).No significant difference of the mRNA expression was found between the low ARMs patients and intermediate ARMs patients (P＞0.05).The protein expressions of Wnt2,Wnt5b and Wnt9a in these patients changed in the same manners with what were found in the mRNA studies,in which Wnt2,Wnt5b and Wnt9a were decreased in the stenotic intestine of HD patients and high ARMs patients.Conclusions The expression of Wnt2,Wnt5b and Wnt9a changes in the tissue samples from the patients with HD and ARMs,which may play a role in the pathogenesis of congenital malformation of the alimentary tract. Key words: Hirschsprung disease; Anus,abnormalities; Rectum,abnormalities; Blotting,western