The expressions of PTEN and PDCD4 in nephroblastoma

Abstract

Objective To investigate the mRNA and protein expressions of PTEN and PDCD4 in nephroblastorna, and to analyze the correlation between their expressions and the clinicopathological factors in nephroblastoma. MethodsThe mRNA and protein expressions of PTEN and PDCD4 in 39nephroblastoma tissues and 15 adjacent nephroblastoma tissues (control group, located 2 cm away from the borderline of the tumor) were studied by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The results were then analyzed with clinical data. Results The mRNA expressions of PTEN and PDCD4 in all 39 nephroblastoma tissues were down-regulated,and the rnRNA OD rations of PTEN and PDCD4 in the control group were twice of those in nephroblastoma tissues. The protein expressions of PTEN and PDCD4 in nephroblastoma tissues (positive rate: 17. 95％, 33. 33％) were significantly lower (P＜0. 05) than those in the control group (positive rate: 93.33％、 100％).The down-regulation of the protein expressions of PTEN and PDCD4 in nephroblastorna tissues were closely related to multiple clinicopathological factors (P＜0. 05), such as age, histopathological and clinical grading, and lymph node metastasis. The gender had no significant impact on repressing the expressions of PTEN and PDCD4 in nephroblastoma tissues. A positive correlation was found between the protein expression of PTEN and that of PDCD4 in nephroblastoma tissues through regression analysis (r= 0. 88, P＜0. 05). ConclusionsThe mRNA and protein expression of PTEN and PDCD4 are significantly decreased in nephroblastoma tissues, and PTEN and PDCD4 may become a reference index to evaluate the biological behavior of the nephroblastoma. Key words: Nephroblastoma; PTEN phosphohydrolase; Genes, tumor suppressor