Abstract
Objective To investigate the expression of E-cadherin and Ezrin in the primary gastric carcinomas versus lymph nodal metastases.and elucidate the relationship between these molectries and invasion and metastasis of gastric adenocareinoma.Methods Eighty paraffin-embedded gastric carcinoma tissue samples together with 40 cases of lymph nodal metastases were obtained.Hematoxylin and eosin stain was used for reviewing and determining the patholoical diagnosis,including histological classification,tumor differentiation.depth of infiltration and nodal status.The expression of E-cadherin and Ezrin was deteeted by means of SP immunohistochemical method.Resuits Normal adjacent gastric mucosa showed intense membranous staining of E-cadherin and Ezrin throughout the epithelium.The staining patterns in carcinoma tissues were different from normal tissues.E-cadherin had membranous and cytoplasmic staining type.Cytoplasmic Ezrin staining was common in tumor ceils.In primary lesions,the expression of E-cadherin and Ezrin was correlated with tumor differentiation.The expression of Ezrin and tlle membranous expression of E-cadherin were reduced in those cases with higher grade of differentiation(P＜0.05;P＜0.01.respectively).In contrast,the cytoplasmic expression of E-cadherin was increased(P＜0.01).The up-regulated cytoplasmic E-cadherin expression was associated with depth of infiltration and lymph node metastatic condition(P＜0.01;P＜0.05,respectively).The cytoplasmic expression level of E-cadherin was higher in primary tumor than in metatstases(P＜0.01).In lymph nodal metastases,the membranous expression of E-cadherin was suppressed in late stage of lymph node metastasis(P＜0.05).In addition,a positive correlation was found between the expression of Ezrin and the membranous expression of E-cadherin(P＜0.01).Conclusion The anomalOUS expression of E-cadherin leads to reduced cell-cell adhesion,thus plays a pivotal role in gastric cancer cells invasion and metastasis.Ezrin is involved in regulating differcntiation of gastric cancer ceIts and regulates cell-cell adhesion and invasion of gastric adenoearcinoma by interacting with the E.cadherin/eatenin complex. Key words: Gastric carcinoma; Metastasis; Cytoskeleton; Protein expression
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
