Abstract
Lipoxygenases (LOXs) are non-haem iron-containing dioxygenases that catalyse oxygenation of polyunsaturated fatty acids and lipids to initiate the formation of a group of biologically active compounds called oxylipins. Plant oxylipins play important and diverse functions in the cells. In the current study, expression analysis during cucumber development using semi-quantitative RT-PCR revealed that 13 of 23 CsLOX genes were detectable, and were tissue specific or preferential accumulation. In total, 12 genes were found to be differentially expressed during fruit development and have different patterns of expression in exocarp, endocarp and pulp at day 5 after anthesis. The expression analysis of these 12 cucumber LOX genes in response to abiotic stresses and plant growth regulator treatments revealed their differential transcript in response to more than one treatment, indicating their diverse functions in abiotic stress and hormone responses. Results suggest that in cucumber the expanded LOX genes may play more diverse roles in life cycle and comprehensive data generated will be helpful in conducting functional genomic studies to understand their precise roles in cucumber fruit development and stress responses.
Highlights
Lipoxygenases (LOXs; EC 1.13.11.12) are a group of non-heme iron-containing dioxygenases that initiate the degradation of free fatty acids and esterified lipids via various branches of the LOX pathway [1]
In the case of linoleic or linolenic acids, this leads to two possible products, the 9- and 13-hydroperoxy fatty acids, which are rapidly converted either chemically or enzymatically into a group of products collectively called oxylipins, such as traumatin, jasmonic acid (JA), and methyl jasmonate (MeJA) [4]
The Expression Profiling of the Cucumber LOX Genes During Vegetative and According to the previous genome sequencing and bioinformatics analysis, the LOX gene family in cucumber consists of 23 LOX genes [37,38], of which there are nine type-1 LOXs predicted to be 9-LOX, which is a notable expansion in the cucumber genome
Summary
Lipoxygenases (LOXs; EC 1.13.11.12) are a group of non-heme iron-containing dioxygenases that initiate the degradation of free fatty acids and esterified lipids via various branches of the LOX pathway [1]. The LOX proteins play important roles in lipid peroxidation under biotic and abiotic stress, and are involved in a number of developmental stages [2,3]. In the case of linoleic or linolenic acids, this leads to two possible products, the 9- and 13-hydroperoxy fatty acids, which are rapidly converted either chemically or enzymatically into a group of products collectively called oxylipins, such as traumatin, jasmonic acid (JA), and methyl jasmonate (MeJA) [4]. Oxylipins have been implicated in a wide range of important physiological functions, such as signal transduction, biotic or abiotic stress response, development and senescence [5,6,7]. Some oxylipins are commercially important compounds, as they can be utilized as components for fragrances [8,9,10]
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