The Expression Profiles of mRNAs and lncRNAs in Buffalo Muscle Stem Cells Driving Myogenic Differentiation.

Ruimen Zhang,Yingming Wei,Qiang An,Jinling Wang,Chaoxia Zou,Zhengzhong Xiao,Zhuyue Wu,Sufang Yang,Yanfei Deng,Hui Li,Xiaoqing Zhou,Deshun Shi

doi:10.3389/fgene.2021.643497

Ruimen Zhang, Yingming Wei + Show 10 more

Open Access

https://doi.org/10.3389/fgene.2021.643497

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Abstract

Buffalo breeding has become an important branch of the beef cattle industry. Hence, it is of great significance to study buffalo meat production and meat quality. However, the expression profiles of mRNA and long non-coding RNAs (lncRNA) molecules in muscle stem cells (MuSCs) development in buffalo have not been explored fully. We, therefore, performed mRNA and lncRNA expression profiling analysis during the proliferation and differentiation phases of MuSCs in buffalo. The results showed that there were 4,820 differentially expressed genes as well as 12,227 mRNAs and 1,352 lncRNAs. These genes were shown to be enriched in essential biological processes such as cell cycle, p53 signaling pathway, RNA transport and calcium signaling pathway. We also identified a number of functionally important genes, such as MCMC4, SERDINE1, ISLR, LOC102394806, and LOC102403551, and found that interference with MYLPF expression significantly inhibited the differentiation of MuSCs. In conclusion, our research revealed the characteristics of mRNA and lncRNA expression during the differentiation of buffalo MuSCs. This study can be used as an important reference for the study of RNA regulation during muscle development in buffalo.

Highlights

There is an annual increase in the global consumption of beef and it is an indispensable food in our modern society, and the beef cattle industry occupies an increasingly important position in modern agricultural practices (Bonny et al, 2015)
The expression levels of muscle-derived marker molecules, MYOD1, MYOG, and MyHC, increased significantly in DM samples of muscle stem cells (MuSCs) (Figure 1D). These results suggest that the cells obtained were MuSCs with the capability of myogenic differentiation
The proliferation and myogenic differentiation phenotypic characteristics of buffalo MuSCs were compared for the first time, and the expression of mRNAs and long non-coding RNAs (lncRNAs) in these cells were reviewed

Summary

Introduction

There is an annual increase in the global consumption of beef and it is an indispensable food in our modern society, and the beef cattle industry occupies an increasingly important position in modern agricultural practices (Bonny et al, 2015). China urgently needs a viable and thriving beef cattle industry in order to provide its society with larger amounts of high-quality beef (Mwangi et al, 2019; Ornaghi et al, 2020). There is a need for us to conduct research on the growth and meat quality of locally produced beef as well as to explore the potential molecular information of breeding stocks so as to provide reference values for future breeding protocols (Grigoletto et al, 2020).

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