Abstract
The sense and antisense digoxigenin-labeled RNA probes of four genes, Cdc25A, Cdc25B, Sox2 and Mnb, were produced by using SP6 and T7 RNA polymerases, respectively, and in vitro transcription. Expression patterns of the four genes were detected by in situ hybridization in HH (Hamburger and Hamilton) stage 10 chick embryos. In general, expression patterns of the four genes were similar. mRNA of the four genes was mostly restricted to the entire CNS (central nervous system). All were confined to an identical region, neural tube, neural groove and caudal neural plate, corresponding to the notochord or spinal cord, but there was some distinction in specific region or in concentration, for example in somites. The overlap in expression at the same developmental stage in the CNS suggests that the four genes may be functional similar or related in CNS development. Expression patterns of the four genes support specific roles of these regulators in the developing CNS.
Highlights
Cell division cycle (Cdc) genes are required for regulation of cell proliferation
Cell cycle progression is regulated by the cyclin-dependent kinase (CDK) family
Whereas mRNA of all four genes was restricted to the entire central nervous system (CNS), only Cdc25A and Cdc25B had weak staining in somites
Summary
Cell division cycle (Cdc) genes are required for regulation of cell proliferation. Others factors are necessary for cell differentiation events. To understand the mechanisms that underlie this regulated process, greater knowledge of the molecular control of the acquisition of cell proliferation and differentiation is required. Considerable progress has been made in identifying the signals and elucidating the molecular mechanisms that regulate cell proliferation and/or differentiation. It is important to investigate these genes at the transcription level and elucidate regulated factors in relation to each other and their roles in embryo development. Cell cycle progression is regulated by the cyclin-dependent kinase (CDK) family. CDK activity throughout the cell cycle is highly regulated by association with cyclins and with inhibitory proteins (Oogood, 2002).
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