The expression pattern of OsDim1 in rice and its proposed function

Henry Akrofi Doku,Li-Juan Chen,Shu-Xian Gan,Cheng-Yun Li,Dong-Sun Lee,Sang-Gu Kang,Meng-Ting Li,Eui-Ho Park,Li Zhou,Wei Li,Sadia Nadir,Qian Zhu

doi:10.1038/s41598-019-54898-1

Abstract

Development of plant tissues is dependent on numerous factors, including hormone activity, signaling, cell division, and elongation. In plants, Defective Entry into Mitosis 1 (Dim1) homologs are recognized as pivotal in leaf senescence and progress of normal growth, but their role in rice has not been functionally characterized. The findings presented in this paper suggest that OsDim1 is important in early seedling development, pollen tube elongation, and impacts rice yield components. The gene is expressed in the scutellum, endosperm, embryonic root, shoot, pollen grains and tubes, as well as in several organs of the rice flower. According to the present study findings, RNAi mediated knockdown of OsDim1 resulted in phytohormonal imbalance, reduced amylase activity, affected differentiation of embryonic root elongation zone tissues, suppressed embryonic root and shoot growth, and impaired pollen tube elongation. In contrast, overexpression of OsDim1 showed significant growth in embryonic roots and shoots, while it increased culm length, total number of tillers per plant, seed setting rate, and total number of grains per panicle compared to its wild type line. In summary, we propose OsDim1 plays an important role in seedling growth and pollen tube elongation, and has pleiotropic effects on reproductive tissues.

Highlights

Development of plant tissues is dependent on numerous factors, including hormone activity, signaling, cell division, and elongation
WW proteins function as transcription co-activators, whereas LSM-domain containing proteins play a role in mRNA decay pathway[17,18,19,20]
The findings revealed no significant differences in pollen fertility (PF; Figs. 5g, 6a,b) and viability (Fig. 6c,d) between the wild type (WT) and the RNAi transgenic plants

Summary

Introduction

Development of plant tissues is dependent on numerous factors, including hormone activity, signaling, cell division, and elongation. The findings presented in this paper suggest that OsDim[1] is important in early seedling development, pollen tube elongation, and impacts rice yield components. We propose OsDim[1] plays an important role in seedling growth and pollen tube elongation, and has pleiotropic effects on reproductive tissues. Failure of Dim[1] to play the role of typical TRXs despite its involvement in disulfide bonds has been attributed to the lack of Cys[79] conservation, the diminished cell environment, and the reduced stability of the protein in the presence of disulfide[9,10]. This article further suggests that OsDim[1] plays a pleiotropic role in rice reproductive tissues and impacts the grain yield components. The findings yielded by the present study improves our current understanding of the role of Dim[1] genes in these processes

Methods

Results

Conclusion