The expression of WNT/β-catenin pathway factors on endometrium cells of in-vitro model of embryo implantation

Abstract

ObjectiveTo explore the role of Wnt/β-catenin pathway in the procedure of embryo implantation.DesignProspective experimental study.Materials and MethodsAfter established in-vitro model of embryo implantation by co-culturing human blastocysts with human endometrial decidualization cell monolayers, β-catenin was detected by immunofluorescence analysis on endometrial cells at pre-, peri- and post-implantation stages. β-catenin,Wnt4,Frz6 were detected by real time fluorescent quantitative PCR analysis on endometrial cells at pre-,peri- and post-implantation stages.ResultsThe blastocysts adhered to the decidualization cell layer within 5-10h, attached and invasion into the decidualization cell layer after 48h of coculture. The expression of β-catenin protein was observed on endometrial cells and mainly located at the cellular membrane at pre-implantation stage; more β-catenin protein was observed at the membrane and cytoplasm on decidualization cells at peri-implantation stage; the expression of β-catenin was detected in cellular nucleus after 48 h of implantation. The expression of β-catenin,Wnt4,Frz6 mRNA was significantly increased on decidualization cells at peri -implantation stage compared with pre-implantation stage (P<0.05), and significantly increased at post-implantation stages compared with peri-implantation stage (P<0.05).ConclusionHuman blastocysts co-cultured with human endometrial decidualization cell monolayers could be used to study the mechanism of embryo implantation. Wnt/β-catenin pathway was activated and played an important role in the procedure of embryo implantation. ObjectiveTo explore the role of Wnt/β-catenin pathway in the procedure of embryo implantation. To explore the role of Wnt/β-catenin pathway in the procedure of embryo implantation. DesignProspective experimental study. Prospective experimental study. Materials and MethodsAfter established in-vitro model of embryo implantation by co-culturing human blastocysts with human endometrial decidualization cell monolayers, β-catenin was detected by immunofluorescence analysis on endometrial cells at pre-, peri- and post-implantation stages. β-catenin,Wnt4,Frz6 were detected by real time fluorescent quantitative PCR analysis on endometrial cells at pre-,peri- and post-implantation stages. After established in-vitro model of embryo implantation by co-culturing human blastocysts with human endometrial decidualization cell monolayers, β-catenin was detected by immunofluorescence analysis on endometrial cells at pre-, peri- and post-implantation stages. β-catenin,Wnt4,Frz6 were detected by real time fluorescent quantitative PCR analysis on endometrial cells at pre-,peri- and post-implantation stages. ResultsThe blastocysts adhered to the decidualization cell layer within 5-10h, attached and invasion into the decidualization cell layer after 48h of coculture. The expression of β-catenin protein was observed on endometrial cells and mainly located at the cellular membrane at pre-implantation stage; more β-catenin protein was observed at the membrane and cytoplasm on decidualization cells at peri-implantation stage; the expression of β-catenin was detected in cellular nucleus after 48 h of implantation. The expression of β-catenin,Wnt4,Frz6 mRNA was significantly increased on decidualization cells at peri -implantation stage compared with pre-implantation stage (P<0.05), and significantly increased at post-implantation stages compared with peri-implantation stage (P<0.05). The blastocysts adhered to the decidualization cell layer within 5-10h, attached and invasion into the decidualization cell layer after 48h of coculture. The expression of β-catenin protein was observed on endometrial cells and mainly located at the cellular membrane at pre-implantation stage; more β-catenin protein was observed at the membrane and cytoplasm on decidualization cells at peri-implantation stage; the expression of β-catenin was detected in cellular nucleus after 48 h of implantation. The expression of β-catenin,Wnt4,Frz6 mRNA was significantly increased on decidualization cells at peri -implantation stage compared with pre-implantation stage (P<0.05), and significantly increased at post-implantation stages compared with peri-implantation stage (P<0.05). ConclusionHuman blastocysts co-cultured with human endometrial decidualization cell monolayers could be used to study the mechanism of embryo implantation. Wnt/β-catenin pathway was activated and played an important role in the procedure of embryo implantation. Human blastocysts co-cultured with human endometrial decidualization cell monolayers could be used to study the mechanism of embryo implantation. Wnt/β-catenin pathway was activated and played an important role in the procedure of embryo implantation.