The expression of von willebrand factor(vWF) and tissue factor(TF) in ultrasound combined with urokinase and RGDS-targeted microbubbles for recanalization of occluded rabbit femoral artery

Abstract

Objective To explore the express of von willebrand factor(vWF) and tissue factor(TF) in ultrasound combined with urokinase and RGDS-targeted microbubbles for recanalization of occluded rabbit femoral artery. Methods A total of 42 rabbits with platelet-rich thrombi in the femoral artery were randomized into 7 treatment groups (n=6 in each group): ①ultrasound alone (US) group; ②ultrasound, non-targeted microbubbles (US+ M) group; ③urokinase alone (UK) group; ④ultrasound, non-targeted microbubble and urokinase (US+ M+ UK) group; ⑤ultrasound, platelet-targeted microbubble (US+ R) group; ⑥platelet-targeted microbubble, urokinase (R+ UK) group; ⑦ultrasound, platelet-targeted microbubble and urokinase (US+ R+ UK) group. A total of 6 ml of infusion liquor of urokinase, RGDS and microbubbles (SonoVue) were mixed by the direct conjugation method, infusion via vein within 20 min. Ultrasound was conducted to lyse the clot for 30 min. The recanalization and the velocity tracing change of blood flow in thrombolytic process and the express of vWF and TF were evaluated at 120 min post treatment. Results For US, UK, US+ M, US+ R and US+ M+ UK groups, recanalization were failed. The R+ UK and US+ R+ UK groups were partly recanalizated or completed recanalized (P<0.001). vWF and TF were positive in US, US+ M, US+ R and R+ UK group. vWF and TF were negative in US+ M+ UK, UK and US+ R+ UK groups. Conclusions The combined effects of low frequency ultrasound and targeted microbubble combined with urokinase can inhibit the expression of vWF and TF, and then promote the thrombolysis, however it can affect the expression of vWF and TF after reperfusion. Key words: Ultrasonography; Targeted microbubbles; Thrombolytic therapy