Abstract
Purpose: To discuss the expression of vascular endothelial growth factor C (VEGF-C) after keratoplasty in rats. Methods: The Fischer 344 rat corneas were transplanted into Lewis rat eyes. The expression of VEGF-C in cornea was examined by immunohistochemistry and real-time PCR. The association of corneal lymphangiogenesis, corneal inflammation, and rejection score (RS), with VEGF-C in transplanted corneas was also examined. Results: Both VEGF-C protein and mRNA upregulated dramatically in rat transplanted corneas. The immunoreactivity was at peak on the 3rd day and the 14th day after keratoplasty, respectively. VEGF-C mRNA was strongly and positively correlated with inflammatory index (IF) (r = 0.93; p < 0.01), lymphatic vessel counting (LVC) (r = 0.40; p < 0.05), and RS (r = 0.50; p < 0.05). The development of VEGF-C mRNA, IF, and RS was almost parallel in the second week after keratoplasty. Conclusions: VEGF-C was expressed in rat transplanted corneas. The expression of VEGF-C correlated closely with corneal inflammation, corneal lymphangiogenesis, and allograft rejection.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
