Abstract
Following fusion of protoplasts from crown gall tumour calli, characterized by hormone independent growth, and protoplasts from normal tissues of a streptomycin-resistant mutant, SR1, we selected hormone independent streptomycin-resistant calli in Nicotiana tabacum. The tumour line, B6S3, lost the ability to form shoots. Some of the selected lines, similar to SR1, however, are morphogenic. Both calli and shoots contained the tumour specific enzyme lysopinedehydrogenase. The hybrid shoots are resistant to Agrobacterium infection and do not root. These tumorous properties are dominantly expressed in the somatic hybrids.
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