The expression of TCRP1 in chronic myeloid leukemia cells and its effects on cell proliferation

Abstract

Objective To investigate the effects of tongue cancer resistance-associated protein 1 (TCRP1) in proliferation of chronic myeloid leukemia cells (CML), and explore the new thoughts of pathogenesis of CML. Methods The expression of TCRP1 was detected in the peripheral blood mononuclear cells (PBMC) of CML with real-time quantitative polymerase chain reaction (PCR) and Western blot. After the expression of TCRP1 was interfered in K562 cells, the proliferation of cells was detected by 3-(4, 5-dimenthylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and soft agar colony forming assay, and the expression of protein kinase B (AKT) and its phosphorylation were tested by Western blot. Results In PBMC of CML patients, the mRNA and protein levels of TCRP1 were significantly higher than those of normal controls. The results of MTS assay and soft agar colony forming assay showed that the proliferation of K562 cells was significantly decreased after the expression of TCRP1 was interfered. After knockdown of TCRP1 in K562 cells, the phosphorylation of AKT was significantly decreased while the expression of total AKT did not change. Conclusions The expression of TCRP1 was increased in CML cells. High expression of TCRP1 might contribute to proliferation of K562 cells via the phosphorylation of AKT. Key words: Oncogenes; Leukemia, myeloid; Cell proliferation; Tongue neoplasms