The expression of progesterone receptor coregulators mRNA and protein in corpus luteum and endometrium of cows during the estrous cycle

Abstract

The aim of this study was to examine whether changes in the mRNA and protein expression of the progesterone receptor (PGR) coactivator P300/CBP-associated factor (PCAF) and the corepressor Nuclear Receptor Corepressor 1 (NCOR1) may participate in the regulation of PGR function during the estrous cycle in corpus luteum (CL) and endometrium and thus modulate the effect of progesterone (P4) within the reproductive system. The experimental material included CL and endometrial tissues from cows on days 2–5, 6–10, 11–16, and 17–20 of the estrous cycle. The mRNA expression of PCAF and NCOR1 was determined by means of real-time PCR, and protein levels were determined using western blotting. The highest mRNA and protein expression for PCAF (P<0.01) and NCOR1 (P<0.01) was found on days 6–16 in CL, whereas mRNA and protein expression for PCAF in endometrium was the highest on days 2–10 (P<0.05), but for NCOR1 it was the highest on days 2–5 (P<0.05) and decreased thereafter. Significant correlations were found between PCAF and NCOR1 mRNA and protein in CL and endometrium, between PCAF mRNA or protein and P4 levels only in CL, and between NCOR1 protein and P4 levels in endometrium only. Correlations between PCAF and NCOR1 mRNA and PCAF and NCOR1 protein were also found. These data suggest that the variable expression of these coregulators in CL and endometrium during the estrous cycle may depend on the influence of P4, and in these tissues both coregulators may compete for binding to the PGR.