Abstract
Purpose: To explore the expressions of p53 and phosphorylation-H2AX in varicocele-induced rat testes. Materials and Methods: A total of 16 adult male Sprague-Dawley rats underwent an operation; 12 underwent an experimental varicocele and 4, as controls, were sham-operated. Groups of 4 varicocele-induced rats underwent a left orchiectomy after 2 or 3 weeks, or both orchiectomies after 4 weeks. The sham-operated rats underwent both orchiectomies after 4 weeks. Sections of both testes from each animal were studied. The changes in the expressions of p53 and phosphorylation of H2AX were determined using immunohistochemistry and western blot. Results: Immunohistochemical staining of the left testes in the varicoceleinduced rats showed that the expressions of p53 and phosphorylation of H2AX had not begun 2 weeks postoperatively, but remarkable results were observed after 3 and 4 weeks. Both testes of the varicocele-induced rats showed the expressions of p53 and phosphorylation of H2AX after 4 weeks, with the left testes being more distinctive in immunohistochemical staining compared to the right. Western blot of the left testes in the varicoceleinduced rats also showed unclear expressions of p53 and γ-H2AX after 2 weeks. Considerable distinction was seen after 3 and 4 weeks compared to the control group. Conclusions: Our results suggest that experimental varicocele is associated with increased sperm DNA damage. These changes may be related to abnormal spermatogenesis. (Korean J Urol 2006;47:1354-1360) ꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏꠏ
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