The expression of miR-125b, miR-9 and miR-22 oncomiRs in exosomes derived from Jurkat and NB4 cell lines

Abstract

Exosomes are nanoparticles released by many types of normal and cancer cells into environment including blood and intracellular fluid. Exosomes have potential ability to be published and be circulated in the body fluid, and its structure allows them to carry cargo well to the target cell. The presence of various bioactive molecules of growth factors, types of signaling molecules, DNA, mRNA and sequences of micro-RNA(miRNA), in content of exosome’s cargo are clearly demonstrated. Here, we evaluated presence of miR-125b, miR-9, miR-22 oncomiRs in the exosomes derived from myeloid and lymphoid cell lines.The exosomes of Jurkat and NB4 cell lines, that lymphoid and myeloid were representative was assessed by Real-Time PCR. The results showed that miR-125b, miR-9 and miR-22 oncomiRs, was expressed in Jurkat and NB4 cells, and also in their exosomes. But the presence of miR-22 in exosomes derived from Jurkat was not detected. Regarding to current findings namely presence of oncomiRs in exosomes-derived cancer cells, more studies recommended to evaluate probable oncogenesis in exosomes- receiving cells. Also, the application of exosomes from normal cell to induce apoptosis in leukemic cells could be verified.