The expression of human neuron-specific enolase gene in human tumor cells and clinical significance

Abstract

Objective To examine the expression of NSE in non-endocrine tumor cells on the levels of transcription, protein and cells.Methods The Reverse transcription-polymerase chain reaction (RT-PCR) with NSE specific primers, Western blot and immunocytochemistry(ICC) were used to check the NSE expression in the tumor cells.Results The NSE expressions in human melanoma line WM451 and WM 983A, human high potential metastasis prostate cancer cell PC-3M and low potential metastasis cancer cell PC-3, breast cancer cell cline MCF-7, colorectal adenocarcinoma cell line HT-29 and SL180, and gastric cancer cell line MGC-803 were found on the levels of transcription, protein and cells. The positive rate was 100%.Conclusions The NSE may be expressed in wider tumors in addition to tumors from neuroendocrine origin, it is worthy of futher studying of it.