The expression of hsa-miR-28-3p and ADAM12 in cord blood mononuclear cells of preterm birth infants

Abstract

Objective Preterm birth is a live birth delivered before 37 weeks of gestation.Preterm birth rates have risen in recent years, and preterm birth is the leading cause of perinatal morbidity and mortality worldwide.Both environmental and genetic factors likely play important roles in the development of preterm birth.MicroRNAs(miRNAs) are 18 to 25 nucleotides, single stranded non-coding RNAs that regulate a wide range of biological processes in development and human disease.In this study, we have investigated the differential expression of hsa-miR-28-3p and ADAM12 in cord blood mononuclear cells of preterm birth and term birth. Methods The cord bloods were collected from the patients of Putuo District Institute of Maternity and Child Health.The preterm group(30 patients) was the patients with spontaneous preterm delivery, and the control group(30 patients) was the patients delivered normal infants at term.The expression levels of hsa-miR-28-3p and ADAM12 mRNA were directly generated by real-time PCR. Results The gestational age and birth weight of preterm group was(30.92±1.73) weeks and(1 646±357)g, and the control group was(39.73±0.58) weeks and(3 301±394)g.The hsa-miR-28-3p expression of preterm group(1.03±0.23) was significantly lower than that of control group(2.32±0.52) in the peripheral blood mononuclear cells(P<0.01); the ADAM12 mRNA expression of preterm group(0.037 8±0.005 6) was significantly higher than that of control group(0.027 6±0.003 9)(P<0.05); the hsa-miR-28-3p expression was significantly correlated with the ADAM12 mRNA expression(r=-0.634 1, P<0.01). Conclusion The lower hsa-miR-28-3p expression of cord blood mononuclear cells may up-regulate ADAM12 mRNA expression, and promotes the occurrence of spontaneous preterm birth. Key words: Preterm birth; MicroRNA; hsa-miR-28-3p; ADAM12,