The expression of dopa decarboxylase and dopamine beta hydroxylase and their responding to bacterial challenge during the ontogenesis of scallop Chlamys farreri

Abstract

Dopa decarboxylase (DDC) and dopamine beta hydroxylase (DBH) is responsible for the synthesis of dopamine and norepinephrine, respectively. In the present study, dopa decarboxylase (CfDDC) and dopamine beta hydroxylase (CfDBH) were selected as indicator to investigate the development of catecholaminergic nervous system in the larvae of scallop Chlamys farreri. The CfDDC and CfDBH transcripts were all detectable during the whole ontogenesis expect for the CfDDC transcripts in 2-cell embryos stage. The expression level of CfDDC and CfDBH mRNA increased significantly in the veliger stage, and reached the peak in late (35.64-fold, P < 0.05) and mid-veliger (400.21-fold, P < 0.05) larvae, respectively. By immunofluorescence, two CfDDC immunoreactive areas were observed in the trochophore and D-hinged larvae, and then three CfDDC immunoreactive areas and two immunopositive fibres formed in early and late veliger larvae, respectively. Two CfDBH immunopositive fibers appeared initially in the early D-hinged stage, and another two similar fibers developed in the late D-hinged stage. The bacteria Vibrio anguillarum challenge could induce the mRNA expression of CfDDC and CfDBH in different developmental stage. The significantly increase of CfDDC mRNA was observed in the trochophore larvae at 12 h (8.61-fold, P < 0.05) and in late D-hinged larvae at 24 h (1.56-fold, P < 0.05) post challenge. The expression level of CfDBH mRNA decreased significantly in late D-hinged larvae at 6 h (0.45-fold, P < 0.05), whereas it increased significantly in late veliger larvae at 12 h after bacterial challenge (14.52-fold, P < 0.05). These results concluded that the scallop catecholaminergic nervous system appeared firstly as the form of dopaminergic neurons in the trochophore larvae, and the developing catecholaminergic nervous system in the trochophore, D-hinged and veliger larvae of scallop could respond to the immune stimulation in different patterns.