Abstract

Objective To explore the effect of maternal deprivation on the expression of dopamine receptor (DRD2) in adult rats' nucleus accumbens and whether DNA methylation was involved in its expression regulatory mechanism.Methods Sixteen newborn rats were randomly divided into two groups,the maternal deprivation group ( n = 8 ) were deprived from their mother 6 hours per day during lactation,while the controls (n =8) without any treatment.When the rats grew up to twelve weeks, DRD2, DNAmethyltransferase mRNA expression level in nucleus accumbens was detected with real-time RTPCR.Results The expression level of DRD2 mRNA in maternal deprivation rats (1.25 ±0.06 ) was significantly lower than that in controls (1.15 ± 0.07) (P 0.05] were not significantly different between both the groups.Conclusion DNA methylation may not be involved in the expression of gene DRD2. Key words: Matenal deprivation; Receptors, Dopamine; DNA methylation; Nucleus accumbens; R-T PCR

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