The expression of bcl-2 and cyclinD1 mRNA in bladder transitional cell carcinoma

Abstract

Objective To investigate the expression and clinical significance of bcl-2 and cyclinD1 mRNA in bladder transitional cell carcinoma(TCCB). Methods The expression of bcl-2 and cyclinD1 mRNA were detected by RT-PCR in 40 patients with TCCB and 10 normal bladder tissues. Results The expressional level of bcl-2 and cyclinD1 mRNA in 40 TCCB tissues(0.650±0.018,0.660±0.022) were significantly higher than that level in normal tissues(0.250±0.035,0.320±0.028) (P<0.05). The expression of bcl-2 mRNA in the TCCB tissues of clinical stage Tα-T2a was higher than the expression in stage T2b-T4(P<0.05). But cyclinD1 mRNA in the TCCB tissues of clinical stage T2b-T4 was higher than the expression in stage Tα-T2a(P<0.05),and cyclinD1 mRNA and bcl-2 had correlation(P<0.05). Conclusions RT-PCR assay is reliable and sensitive for detecting bcl-2 and cyclinD1 mRNA in carcinoma specimens from TCCB. The high expression of bcl-2 and cyclinD1 mRNA were correlated to the malignancy. Key words: bcl-2 ; cyclinD1 ; Bladder transitional cell carcinoma ; Reverse transcription polymerase chain reaction ;