The Expression Inhibition of HnRNP B1 in Lung Cell Line A549 by Small Interfering RNA

Abstract

Background: Preliminary studies indicate a relative link between the expression of heterogeneous nuclear ribonucleoproteins (hnRNPs) and cancer. Overexpression of hnRNP B1 correlated with the eventual development of lung cancer. Here we describe inhibition of HnRNP B1 replication in human lung cell line A549 by siRNAs targeted to various regions of hnRNP B1 sequences. Methods: Small interfering RNA technique was used to interfer the expression of hnRNPB1 in Lung Cell Line A549 in vitro; the RT-PCR and Western blot technique were used to detect the quantity of hnRNPB1 mRNA and its protein; flow cytometry was used to detect the apoptosis; then the cells were transfected to nude mice. Results: We demonstrated that plasmidderived siRNAs could inhibit hnRNP B1 mRNA and protein expression, and the siRNA expressed from activated plasmid in A549 cells could suppress the cell proliferation by increasing the G1 cells. Our experiment also found that siRNA could induce apoptosis. Conclusions: These results demonstrated the effectiveness of siRNA in lung cancer cells, which indicated a possible clinical therapy method for lung cancer development.