Abstract
The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF) genes, initially characterized as nitrate or peptide transporters in plants, are involved in the transport of a large variety of substrates, including amino acids, nitrate, auxin (IAA), jasmonates (JAs), abscisic acid (ABA) and gibberellins (GAs) and glucosinolates. A total of 169 potential functional NPF genes were excavated in Brassica napus, and they showed diversified expression patterns in 90 different organs or tissues based on transcriptome profile data. The complex time-serial expression changes were found for most functional NPF genes in the development process of leaves, silique walls and seeds, which indicated that the expression of Brassica napus NPF (BnaNPF) genes may respond to altered phytohormone and secondary metabolite content through combining with promoter element enrichment analysis. Furthermore, many BnaNPF genes were detected to respond to vernalization with two different patterns, and 20 BnaNPF genes responded to nitrate deficiency. These results will provide useful information for further investigation of the biological function of BnaNPF genes for growth and development in rapeseed.
Highlights
Based on BLASTP using 53 Arabidopsis NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF) protein and phylogenetic analysis (Figure S1), a total of 169 NPF genes encoding 186 proteins were identified in the B. napus genome
The results show that 157 Brassica napus NPF (BnaNPF) genes contained at least one type of Cis-Acting Regulatory Elements (CREs) in the promoter regions, which indicated that complex transcriptional regulation might be implicated for BnaNPF genes
Total of 169 BnaNPF genes coding 186 proteins were identified in the B. napus genome in this study and designated as BnaNPF1.1 to BnaNPF8.19 in eight subfamilies based on phylogenetic analysis, and they exhibited evolutionary and syntenic relationships with
Summary
Received: 15 April 2021Accepted: 3 May 2021Published: 6 May 2021Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Licensee MDPI, Basel, Switzerland.Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).Plant NPF (NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY) proteins display sequence homology with the proton-coupled oligopeptide transporter (POT) family of peptide transporters, belong to the large peptide transporter (PTR) family [1] and are involved in dietary nitrogen absorption in the form of di- and tripeptides [2,3]. In plants, NPF members are initially characterized as nitrate or peptide transporters. AtNPF6.3, known as
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