The expression and significance of Zinc-Alphα2 glycoprotein in serum of ureteral carcinoma patients

Abstract

Objective To screen and identify the differentially expressed proteins of ureteral carcinoma by using surface-enhanced laseresorption/inionation time of flight mass spectromery (SELDI-TOF MS). Methods A total of 20 serum samples from adults with ureteral carcinoma(ureteral carcinoma group), and 20 serum samples from healthy adults(control group)were selected.SELDI-TOF MS technology was applied to detect the differentially expressed proteins.The differential spot was identified by matrix-assisted laser desorption/ionization tandem time of flight mass spectrometry(MALDITOF/TOF MS). Results Fourteen different proteins peaks were found(P< 0. 01). The highest Youden's index was obtained, selecting one differentially expressed protein with m/z of 4 222 Da. The selected expression protein in control group and ureteral carcinoma group was 12 003. 33± 3 234. 93 and 18 144. 66±2 047. 05 respectively(P< 0. 01).MALDI-TOF/TOF MS was used for identification of the peak spot, and the peak was further identified as Zinc-Alphα2 glycoprotein (ZAG). Conclusion A differentially expressed protein of ZAG of ureteral carcinoma was successfully obtained through proteomics technology.ZAG is expected to be a new marker for the early diagnosis and prognosis of ureteral carcinoma. Key words: Zinc-Alphα2 glycoprotein; Bological markers; Ureteral carcinoma; Proteomics