The expression and significance of the correlation factor of unfolded protein response: Bip in hepatoma

Abstract

Objective To detect the express of immunoglobulin-binding protein (Bip) mRNA in the tissue of hepatoma;demonstrating that it exist activation of unfold protein response (UPR) in process of growth, and the expression of UPR was higher than normal tissue. Methods To study the mRNA of Bip in 42 case example of hepatome, 15 case paired adjacent hepatic tissues to carcinoma and 15 case normal hepatic tissues by RT-PCR. To qualitation check the expression of Bip in the level of protein by Western blotting. Results The express of Bip mRNA in hepatoma tissue、adjacent hepatic tissue to carcinoma and normai hepatic tissue respectively was 0. 4772 ± 0. 0401、0. 4332 ± 0. 0488、0. 4301 ± 0. 0398; the express of Bip mRNA was higher in hepatoma than others (P ＜ 0. 05 ). The experiment indicate: the Bip express in the hepatoma tissue and normal hepatic tissue, but it was higher in hepatoma tissue than in the normal hepatic tissue. Conclusion It exist activation of UPR in process of growth;The express of Bip protern and its mRNA was accordant, it was higher in hepatoma tissue than in normal hepatic tissue. The changes of its gene and protern is possible to have the relationship with the carcinogenesis, growth and transfer of hepatoma. And it may have significant effect in molecule mechanism of hepatoma. It also descripte that unfold protein response may have very important role in the pocess of carcinogenesis. Key words: Hepatoma; Hepatic tissue; Bip; RT-PCR; Western blotting