The Expression and Regulatory Roles of Long Non-Coding RNAs in Periodontal Ligament Cells: A Systematic Review.

Yifan Lin,Yanqi Yang,Lijian Jin,Zhongyuan Tang

doi:10.3390/biom12020304

Yifan Lin, Yanqi Yang + Show 2 more

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https://doi.org/10.3390/biom12020304

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Journal: Biomolecules	Publication Date: Feb 12, 2022
Citations: 4	License type: CC BY 4.0

Affiliation: University of Hong Kong

Abstract

Periodontal ligament (PDL) cells play a pivotal role in periodontal and bone homeostasis and have promising potential for regenerative medicine and tissue engineering. There is compelling evidence that long non-coding RNAs (lncRNAs) are differentially expressed in PDL cells compared to other cell types and that these lncRNAs are involved in a variety of biological processes. This study systematically reviews the current evidence regarding the expression and regulatory functions of lncRNAs in PDL cells during various biological processes. A systematic search was conducted on PubMed, the Web of Science, Embase, and Google Scholar to include articles published up to 1 July 2021. Original research articles that investigated the expression or regulation of lncRNAs in PDL cells were selected and evaluated for a systematic review. Fifty studies were ultimately included, based on our eligibility criteria. Thirteen of these studies broadly explored the expression profiles of lncRNAs in PDL cells using microarray or RNA sequencing. Nineteen studies investigated the mechanisms by which lncRNAs regulate osteogenic differentiation in PDL cells. The remaining 18 studies investigated the mechanism by which lncRNAs regulate the responses of PDL cells to various stimuli, namely, lipopolysaccharide-induced inflammation, tumor necrosis factor alpha-induced inflammation, mechanical stress, oxidative stress, or hypoxia. We systematically reviewed studies on the expression and regulatory roles of lncRNAs in diverse biological processes in PDL cells, including osteogenic differentiation and cellular responses to inflammation, mechanical stress, and other stimuli. These results provide new insights that may guide the development of lncRNA-based therapeutics for periodontal and bone regeneration.

Highlights

Division of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Division of Periodontology and Implant Dentistry, Faculty of Dentistry, The University of Hong Kong, These authors contributed to this work
This study demonstrated the feasible and safe application of autologous Periodontal ligament (PDL) cells for periodontal regenerative treatment in patients diagnosed with periodontitis [19]
The inclusion criteria were as follows: (1) studies based on cell, human, or animal models; (2) studies related to the expression or regulation of long non-coding RNAs (lncRNAs) in PDL cells; and

Summary

Introduction

Division of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Division of Periodontology and Implant Dentistry, Faculty of Dentistry, The University of Hong Kong, These authors contributed to this work. This study systematically reviews the current evidence regarding the expression and regulatory functions of lncRNAs in PDL cells during various biological processes. Fifty studies were included, based on our eligibility criteria Thirteen of these studies broadly explored the expression profiles of lncRNAs in PDL cells using microarray or RNA sequencing. We systematically reviewed studies on the expression and regulatory roles of lncRNAs in diverse biological processes in PDL cells, including osteogenic differentiation and cellular responses to inflammation, mechanical stress, and other stimuli. These results provide new insights that may guide the development of lncRNA-based therapeutics for periodontal and bone regeneration. Introduction with regard to jurisdictional claims in Periodontitis is a plaque-induced inflammatory oral disease that causes the progressive breakdown of periodontal tissue, and it is one of the leading causes of tooth loss [1,2]

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