The expression and clinical significance of microRNA-107 in bladder transitional cell carcinoma

Abstract

Objective To detect the expression level of microRNA(miR)- 107 in the different clinical stages of bladder transitional cell carcinoma(BTCC), and explore the relationship between miR- 107 pathway and clinicopathological features of BTCC. Methods Fifty- six BTCC specimens surgically resected were selected.For each specimen, the cancerous tissue and its remote normal mucosa were analyzed and compared.The expression of miR- 107, let- 7, Dicer, Ras/high mobility group A2(HMGA2), and zinc-finger E-box binding homeobox 1(ZEB1)/zinc-finger E-box binding homeobox 2(ZEB2)in the resected cancer tissues was detected by using real- time quantitative polymerase chain reaction(Real- time PCR), Western blotting and immunohistochemistry, and their correlation with clinicopathological features was analyzed. Results (1) The expression rate of miR-107 was significantly higher in BTCC tissues than in normal tissues(14.983±6.215 vs.9.124±5.595), and that in deeply infiltrating group was significantly higher than that in superficially infiltrating group (15.183±6.035 vs.11.728±5.472, and 14.663±5.215 vs.10.983±5.674,P 0.05).Ras/ HMGA2, let- 7 downstream regulatory factors, were significantly higher in BTCC than in normal tissues(89.3% vs.23.2%, and 80.3% vs.28.6%,P< 0.05). The expression of let- 7, and Dicer in BTCC tissues was significantly lower than in normal bladder epithelium (P< 0.05);(2) miR- 107 expression was inversely correlated to let- 7 and Dicer. Conclusion The miR- 107 was related with the clinical stages of BTCC through the regulation of Dicer and let- 7 pathways, which mediates the occurrence and development of bladder cancer. Key words: Micro RNA- 107; Dicer; Bladder transitional cell carcinoma; Epithelialmesenchymal transition