Abstract
Objective To investigate the expression patterns of FoxO3a and MuRF-1 in the myocytes of denervated gastrocnemius muscle in rats. Methods Seventy-two Wistar rats were randomly divided into 12 groups with 6 rats each. 3＂he fight sciatic nerve was transected while the left underwent a sham operation. One group of rots was sacrificed at postoperative day 0, 1, 2, 3, 4, 5, 6, 7, 10, 14, 21 and 28 respectively to harvest the fight gastrecnemlus muscles. The samples were frozen at - 70℃. Real-time PCR was used to detect the mRNA level of FoxO3a and MuRF- 1, and western blot was done to detect the protein concentration of FoxO3a and MuRF-1 for each sample. Results The mRNA level of MuRF-1 reached its peak at 4 days after the denervation （ 18.12 folds）, and returned to normal level after 14 days. The mRNA level of FoxO3a peaked at 6 days after the denervation （7.06 folds）, while maintaining at a high level despite the slow decline. The protein concentration of FoxO3a and MuRF-1 increased with the days of denervation. Conclusion The expressions of FoxO3a and MuRF-1 are closely related to the process of muscle atrophy after denervation. Both FoxO3a and MuRF-1 can be the targets for drug treatment or gene therapy to delay denervatlon atrophy. Key words: Muscle atrophy; Rats; Denervation; FoxO3a; MuRF-1
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
