Abstract
West Aceh Region has Nypa fruticans mangrove community distributed almost ±100 Ha. N. fruticans leaves have been used as traditional cigarette paper. The objective of this study was to explore bioactive compound of N. fruticans from the coast of West Aceh as antioxidant. The extraction used 3 solvents including methanol (polar), etyl acetate (semi polar), and n-hexane (non polar). The result showed the methanol extract contained bioactive compound content such as phenolic, flavonoid, tannin, saponin, and steroid while the etyl acetate extract comprised phenolic, tannin, and steroid. Moreover, n-hexane extract only had steroid compound. The result of antioxidant activit assay showed etyl acetate extract had IC50 value 8.81 µg/mL, methanol extract had IC50 value 9.31 µg/mL and n-hexane extract was 238.47 µg/mL with ascorbic acid as positive control. It is indicated the presence of phenolic, flavonoid, tannin, and saponin in the ethyl acetate extract play the role as antioxidant.
Highlights
West Aceh Region has Nypa fruticans mangrove community distributed almost ±100 Ha
N. fruticans leaves have been used as traditional cigarette paper
The objective of this study was to explore bioactive compound of N. fruticans from the coast of West Aceh as antioxidant
Summary
Sampel dalam penelitian ini adalah tumbuhan nipah (N. fruticans) yang diperoleh dari Pesisir Peunaga Pasi Aceh Barat. Bahan kimia yang dipakai dalam ekstraksi dan analisis yaitu metanol (Merck), etil asetat (Merck) dan n-heksana (Merck), larutan HCl 2 N (Merck), asam askorbat (Merck), FeCl3 5% (Merck), kupri klorida dua hidrat (Merck), NH3 (Merck), H2SO4 2M (Merck), H2SO4 pekat (Merck), pereaksi Dragendorff (Sigma Aldrich), Meyer (Sigma-Aldrich), Wagner (Sigma-Aldrich), amil alkohol (Merck), FeCl3 10 % (Merck), NaOH 10 % (Merck), CH3COOH (Merck), dietil eter (Merck) dan DPPH (Sigma-Aldrich). Peralatan yang digunakan dalam penelitian ini adalah spektrofotometer UVVIS (Shimadzu 1240), tabung Eppendorf, oven, tanur listrik, vorteks, sonikator, inkubator, eksikator, gelas Erlenmeyer, gelas piala, pipet volumetrik, pipet mikro, cawan petri, dan kertas saring biasa
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