The experimental study on osteogenic potential of goat adipose derived stromal cells induced by PRP with rhBMP-2

Abstract

Objective To evaluate the feasibilty of modified osteogenic culturing of goat adipose derived stromal cells (ADSCs). Methods From March, 2013 to September, 2014, platelet-rich plasma (PRP) was made from goat autogenous vein blood, and abodimoneal fat was aspirated, following aspetic procedure, primary and series passage of ADSCs was established. Osteoinduced ADSCs was carried out according to following group: combinative osteo-induction group (PRP+rhBMP-2+ADSCs) , growth factor group (rhBMP-2+ADSCs) , conventional inductive group (dexamathasone+ascorbic acid + ADSCs) and noninductive group (blank group). Converted microscope was used to observe cellular pattern, cell activity, osteocalcin and collagen type I level were detected at 1, 3, 5, 7, 9, 13, 17, 21 days. Red Alazarin and Von Kossa staining were also assayed at different interval. Results Under observation of converted microscopy, remarkable cell proliferation with abundant ECM was noticed in combinative osteoinduction group, compared with other groups, level of cell activity, osteocalcin, collagen type I [ (0.82 ± 2.19) AU, (79.82 ± 1.36) U/L at, (78.51 ± 4.32) μg/ml at]were significantly higher than other groups (P < 0.05) , and remarkable ALP expression and calcifed nodules were also seen. Conclusion PRP can enhance the inductive effect of rhBMP-2, and combinative osteoinduction procedure acts as a satisfactory culturing method. Key words: Platelet-rich plasma; Adipose derived stromal cells; Osteoinduction; Recombinant human morphogenetic protein-2