Abstract
Objective To observe the effect of JWSNS serum on proliferation and apoptosis hepatic stellate cells.Methods After being added different concentrations of JWSNS (the low concentrations of JWSNS:0.78 g/ml of crude drug; the medium concentration group of JWSNS:1.56 g/ml of crude drug; the high concentration group of JWSNS:3.12 g/ml of crude drug) drug-containing serum in vitro HSC-T6 cells for 12h,24 h and 48 h respectively,detected serum HSC-T6 proliferation with MTT colorimetry method and measured HSC-T6 apoptosis with flow cytometry and TUNEL method.Results (①) After applied JWSNS on rats HSC-T6,the Cell proliferation was inhibited which showed a time-concentration dependence.The differences were significant when comparing each JWSNS group with the control group (P<0.01).High concentration of JWSNS group showed significant difference when compared with Biejiaruangan tablets group (P<0.05) with high concentration of JWSNS (0.399± 0.041) % after 48h,and Biejia-Ruangan tablets (0.429± 0.037) % after 48 h.② Flow cytometry analysis showed each JWSNS group and Biejiaruangan tablets group had significant increased cell apoptosis when compared with the control group (P<0.05) after 12 h,24 h,and 48 h.JWSNS medium concentration group [12 h was (17.83±0.25)%,24 h was (26.06±0.26)%,48 h was (39.30±2.25) %] and JWSNS high concentration group [12 h was (27.15±0.29)%,24 h was (38.96±0.51)%,48 h was (49.34± 0.77) %] had a significant increased cell apoptosis compared to the Biejia-Ruangan tablets group [ 12 h was (8.31 ± 0.30) %,24 h was (16.25 ± 0.25) %,48 h was (27.12± 0.39) %].③ TUNEL detection showed that each concentration of JWSNS group [the low concentration of JWSNS:was (25.1 ± 1.48)%,medium concentration group of JWSNS was(39.30±2.25)%,high concentration group of JWSNS was(39.30±2.25)%] had a significant increased cell apoptosis rate than Biejiaruangan tablets group (30.0± 3.92) after 48 h (P<0.01).Conclusion JWSNS containing serum can inhibit the proliferation of HSC-T6 in vitro,promote the apoptosis Key words: JWSNS; Hepatic stellate cell; Cell proliferation; Cell apoptosis
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