Abstract
Objective To sutdy the expression of B cell lymphoma/leukemia-2 (bcl-2), twist family basic helix-loop-helix transcription factor 1 (Twist1) in oral squamous cell carcinoma and its modulating epithelial-mesenchymal transition (EMT). Methods Immunohistochemistry was used to detect the bcl-2, Twist1 and EMT related proteins expression among 82 OSCC samples and 24 paracancerous samples. The relationship between the bcl-2, Twist1 and the EMT biomarkers was analyzed. Western blotting were used to observe the bcl-2, Twist1 and EMT related proteins expression. Immunoprecipitation were used to assessed interaction between bcl-2 and Twist1. Results bcl-2, Twist1 expression in oral squamous cell carcinoma was significantly higher than the adjacent tissues. Of 82 cases OSCC, bcl-2 overexpression were found in 48 cases (58.64%) and Twist1 overexpraeeion were in 46 cases (56.10%), which were significantly higher than the adjacent tissues [bcl-2: 6 cases (25.00%), P<0.05, Twist1: 4 cases (16.67%), P<0.05]. In poorly differentiated and lymph node metastasis group, the expression of bcl-2, Twist1, N-cadherin, Vimentin were higher than those in high differentiation and non-lymph node metastasis group. Twist1 showed a positive correlation with bcl-2, N-cadherin, Vimentin (correlation coefficients were 0.303, 0.249, 0.348 respectively, P<0.05), but negatively correlated with E-cadherin (correlation coefficient of -0.547, P<0.05). Immunoprecipitation showed that bcl-2, Twist1 can interact with each other. Conclusion bcl-2 and Twist1 overexpressed in oral squamous cell carcinoma and form a complex, bcl-2/Twist1 complex might play a critical role in modulating EMT. Key words: Oral squamous cell carcinoma; B cell lymphoma/leukemia-2; Twist family basic helix-loop-helix transcription factor 1; Epithelial-mesenehymal transition; Lymthatic metastasis
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
