Abstract
The article presents the experience of obtaining hematopoietic stem cells (HSCs) and their immunophenotyping using the method of flow cytometry at the State Institution "Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine", Lviv. Lviv, Ukraine. Hematopoietic stem cells are key elements in the hematopoietic system and are the basis for bone marrow transplantation, which is used to treat various oncohematologic diseases, including leukemia, lymphoma, and others. The results of the analysis of HSCs in different patients with multiple myeloma showed variability in the concentration of HSCs in peripheral blood and in leukoconcentrate at different stages of apheresis. A high concentration of HSCs after stimulation with colony-stimulating factors was noted, as well as a case with no response to stimulation with colony-stimulating factors and the effectiveness of their cryofreezing. The results of immunophenotyping in the presence of HSCs populations at different stages of the differentiation and the experience of determining cell viability after cryofreezing and long-term storage are also presented.
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