Abstract
The following topics are covered in this chapter. Types of exogenous DNA manipulation essential for detecting and understanding mutations, DNA profiling, etc. These include the following. Sequencing any size of DNA, including entire genomes by next-generation sequencing. Cloning using restriction enzymes and a vector to carry a DNA fragment into a host cell for replication. Screening for a required gene. Gene cloning without a vector. Polymerase chain reaction (PCR) for producing many copies from a very small amount of a DNA segment. Emulsion PCR. Vectors vs. PCR cloning.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
