The evolution of solution state NMR pulse sequences through the 'eyes' of triple-resonance spectroscopy.

Abstract

Careful pulse sequence design and optimization is critical to the success of a given NMR experiment. Over the past several decades the level of sophistication of NMR pulse sequences has increased tremendously, leading to large spectral sensitivity and resolution improvements, to data sets with far fewer artifacts, and to much more rapid acquisition times, opening up a wide range of applications. Here I briefly highlight how pulse sequence 'engineering' has evolved, focusing on liquid state NMR, and, in particular, on the HNCA-class of triple-resonance experiment. In many respects, the evolution of triple-resonance NMR mirrors the evolution of solution state NMR experiments in general, with 'tricks' that first appeared in triple-resonance pulse sequences or that were motivated by them now incorporated into a broad range of experiments.