Abstract
The Evaluation of the Speed-Oligo® Mycobacteria Assay for Identification of Mycobacterium spp. from Smear Positive and Negative Sputum Samples
Highlights
Tuberculosis (TB) is a chronic infectious disease that is common all over the World [1]
Nontuberculous mycobacteria (NTM) infections are getting increase depend on a immunosuppression, AIDS, malignancy, drug abuse, bad living conditions or medical intervention
The aim of this study was to evaluate Speed-oligo® Direct Mycobacterium tuberculosis (SO-DMT) by using 25 type reference strains of mycobacteria (18 non-tuberculosis mycobacteria, M. tuberculosis H37Rv and M. tuberculosis H37Ra), 60 sputum samples (40 smear positive, 20 smear negative) collected from patient with suspected TB
Summary
Tuberculosis (TB) is a chronic infectious disease that is common all over the World [1]. Speed-oligo® Direct Mycobacterium tuberculosis (SO-DMT) is an oligochromatographic test (Vircell SL, Santa Fe, Granada, Spain) for the specific identification of M. tuberculosis complex (MTC) from Mycobacterium genus in respiratory specimens. It is based on polymerase chain reaction targeting 16S rRNA and 16S-23S rRNA regions and double-reverse hybridization on a dipstick using probes bound to colloidal gold and to the membrane. It may represent a fast and easy alternative for differentiating between MTBC and NTM in direct samples at laboratories with standard laboratory equipment (thermocycler and thermoblock) [5,6,7,8]. The aim of this study was to evaluate SO-DMT by using 25 type reference strains of mycobacteria (18 non-tuberculosis mycobacteria, M. tuberculosis H37Rv and M. tuberculosis H37Ra), 60 sputum samples (40 smear positive, 20 smear negative) collected from patient with suspected TB
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