The evaluation of enteric viruses in asymptomatic free‐ranging non‐human primates (Alouatta guariba clamitans, Alouatta caraya, Callithrix spp., Callithrix penicillata, and Leontopithecus caissara) in southern Brazil

Abstract

This study evaluated the presence of rotavirus groups A, B, and C (RV-A, RV-B, and RV-C), sapovirus (SaV), and norovirus (NoV) in asymptomatic non-human primates (NHP). Fecal samples were collected from 19 recently captured (Red-howler, Alouatta guariba clamitans, n=18; Howler, Alouatta caraya, n=1) and 43 free-ranging NHP (Marmosets, Callithrix spp., Callithrix penicillata, n=30; Black-faced lion tamarin, Leontopithecus caissara, n=12, Red-howler, Alouatta guariba clamitans, n=1) that were maintained in southern Brazil without manifestation of diarrhea. Screening was performed by a combination of silver-stained polyacrylamide gel electrophoresis (ss-PAGE) and RT-PCR analyses. All samples were negative for RV-A, RV-B, RV-C, SaV, and NoV by both assays. The negative results obtained might be due to the absence of clinical manifestations of disease in the population of NHP evaluated.