Abstract
The digestibility of animal by-product protein was measured using a direct colorimetric assay of protein in feed and excreta samples. Excreta samples were generated by using a modification of the true metabolizable energy procedure (Sibbald, 1979). Dried excreta and feed samples were extracted with .5N NaOH for 2 hr at 45 C in an orbital incubator, and the solubilized protein was quantitated directly using Coomassie brilliant blue dye-binding. Digestibility of meat and bone meal protein by this method ranged between 70.3 and 96.0%. The digestibility of feather meals ranged between 79.0 and 86.1%. Coomassie brilliant blue reacts specifically with intact protein molecules and is insensitive to uric acid nitrogen. This procedure may prove to be especially useful in the evaluation of protein digestibility in the chicken.
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